AffiELISA® Human UBE2I Kit: Super-Sensitive Ubiquitin Conjugating Enzyme E2I ELISA Detection

The AffiELISA® Human UBE2I Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2I (UBE2I) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2I (UBE2I) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Ubiquitin Conjugating Enzyme E2I (UBE2I) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Ubiquitin Conjugating Enzyme E2I (UBE2I) in the samples can be determined.