The AffiELISA® Human VEGF121 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Vascular Endothelial Growth Factor 121 (VEGF121) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor 121 (VEGF121) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor 121 (VEGF121) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Vascular Endothelial Growth Factor 121 (VEGF121) in the samples can be determined.
AffiELISA® Human VEGF121 Kit
Vascular Endothelial Growth Factor 121
Vascular Endothelial Growth Factor 121
Human
Sandwich ELISA
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Cytokine, Signal transduction, Tumor immunity, Infection immunity.