The AffiELISA® Human VIP Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Vasoactive Intestinal Peptide (VIP) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Vasoactive Intestinal Peptide (VIP) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Vasoactive Intestinal Peptide (VIP) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Vasoactive Intestinal Peptide (VIP) in the samples can be determined.
AffiELISA® Human VIP Kit
Vasoactive Intestinal Peptide
PHM27 - Intestinal peptide PHV-42 - Peptide histidine valine 42 - Peptide histidine methioninamide 27
Human
Sandwich ELISA
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Endocrinology, Gastroenterology, Hormone metabolism.