The AffiELISA® Human VISA Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Virus Induced Signaling Adapter (VISA) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Virus Induced Signaling Adapter (VISA) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Virus Induced Signaling Adapter (VISA) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Virus Induced Signaling Adapter (VISA) in the samples can be determined.
AffiELISA® Human VISA Kit
Virus Induced Signaling Adapter
CARDIF - IPS1 - MAVS - Mitochondrial Antiviral-Signaling Protein - Interferon beta promoter stimulator protein 1 - CARD Adaptor Inducing IFN-beta - NF-kappa-B-activating protein 031N
Human
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids.
Infection immunity.