AffiELISA® Pan VB1 Kit: Max Sensitivity Vitamin B1 ELISA Detection

The AffiELISA® Pan VB1 Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Vitamin B1 (VB1) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Vitamin B1 (VB1) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Vitamin B1 (VB1) in the samples can be determined.