The AffiELISA® Pan VD3 Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Vitamin D3 (VD3) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Vitamin D3 (VD3) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Vitamin D3 (VD3) in the samples can be determined.
AffiELISA® Pan VD3 Kit
Vitamin D3
Cholecalciferol - Toxiferol - Activated 7-Dehydrocholesterol
Pan
Competitive Inhibition ELISA
Serum, plasma and other biological fluids
Metabolic pathway, Reproductive science, Nutrition metabolism, Bone metabolism.