The AffiELISA® Human WNT9B Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 9B (WNT9B) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 9B (WNT9B) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 9B (WNT9B) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Wingless Type MMTV Integration Site Family, Member 9B (WNT9B) in the samples can be determined.
AffiELISA® Human WNT9B Kit
Wingless Type MMTV Integration Site Family, Member 9B
WNT15 - WNT14B
Human
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids
Signal transduction.