The AffiELISA® Human XRCC5 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to X-Ray Repair Cross Complementing 5 (XRCC5) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to X-Ray Repair Cross Complementing 5 (XRCC5) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain X-Ray Repair Cross Complementing 5 (XRCC5) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of X-Ray Repair Cross Complementing 5 (XRCC5) in the samples can be determined.
AffiELISA® Human XRCC5 Kit
X-Ray Repair Cross Complementing 5
KARP1 - KU80 - KUB2 - Ku86 - NFIV - TLAA - Lupus Ku Autoantigen Protein P80 - Nuclear factor IV - 86 kDa of Ku antigen - CTC box-binding factor 85 kDa - Thyroid-lupus autoantigen
Human
Sandwich ELISA
Tissue homogenates and other biological fluids.
Tumor immunity.