The AffiELISA® Human YBX1 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Y-Box Binding Protein 1 (YBX1) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Y-Box Binding Protein 1 (YBX1) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Y-Box Binding Protein 1 (YBX1) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Y-Box Binding Protein 1 (YBX1) in the samples can be determined.
AffiELISA® Human YBX1 Kit
Y-Box Binding Protein 1
BP8 - CSDA2 - CSDB - DBPB - MDR-NF1 - NSEP1 - YB1 - Nuclease Sensitive Element Binding Protein 1 - CCAAT-binding transcription factor I subunit A - DNA-binding protein B
Human
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids
Tumor immunity.