Experience the next level of biotechnology research with AffiELISA® kits. Our high-quality kits deliver exceptional accuracy, sensitivity, and versatility for a wide range of applications. Streamline your workflow and rely on our uncompromising quality to drive meaningful results. With expert support and resources, AffiELISA® is your trusted partner in advancing scientific discovery. Explore and buy our AffiELISA® Human gABA (Gamma-Aminobutyric Acid) ELISA Kit.
14.6 pg/mL
2000 pg/mL
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
2.5h
Signal transductionCardiovascular biologyNeuro scienceBone metabolism
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human gABA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human gABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human gABA in the samples is then determined by comparing the OD of the samples to the standard curve.
N/A