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0.048 ng/mL
10 ng/mL
Serum, plasma, tissue homogenates and other biological fluids.
3.5h
Enzyme & KinaseEndocrinologyNeuro scienceAutoimmunity
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-Sulfatide protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-Sulfatide. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-Sulfatide in the samples is then determined by comparing the OD of the samples to the standard curve.
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