Experience the next level of biotechnology research with AffiELISA® kits. Our high-quality kits deliver exceptional accuracy, sensitivity, and versatility for a wide range of applications. Streamline your workflow and rely on our uncompromising quality to drive meaningful results. With expert support and resources, AffiELISA® is your trusted partner in advancing scientific discovery. Explore and buy our AffiELISA® Human BUB1B (Mitotic Checkpoint Serine/threonine-Protein Kinase BUB1 Beta) ELISA Kit.
0.12 ng/mL
20 ng/mL
serum, plasma and other biological fluids
3.5h
Enzyme & Kinase
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BUB1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BUB1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BUB1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BUB1B in the samples is then determined by comparing the OD of the samples to the standard curve.
N/A