Experience the next level of biotechnology research with AffiELISA® kits. Our high-quality kits deliver exceptional accuracy, sensitivity, and versatility for a wide range of applications. Streamline your workflow and rely on our uncompromising quality to drive meaningful results. With expert support and resources, AffiELISA® is your trusted partner in advancing scientific discovery. Explore and buy our AffiELISA® Human 25-OH-D (25 Hydroxy Vitamin D) ELISA Kit.
1.41 ng/mL
200 ng/mL
serum, plasma and other biological fluids
2.5h
Reproductive scienceGenetic scienceNutrition metabolismBone metabolism
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
N/A