Experience the next level of biotechnology research with AffiELISA® kits. Our high-quality kits deliver exceptional accuracy, sensitivity, and versatility for a wide range of applications. Streamline your workflow and rely on our uncompromising quality to drive meaningful results. With expert support and resources, AffiELISA® is your trusted partner in advancing scientific discovery. Explore and buy our AffiELISA® Horse ADP (Adiponectin) ELISA Kit.
0.051 μg/mL
10 μg/mL
Serum, plasma and other biological fluids
3.5h
Metabolic pathwayInfection immunityCardiovascular biology
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse ADP in the samples is then determined by comparing the OD of the samples to the standard curve.
N/A