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26.29 pg/mL
6000 pg/mL
serum, plasma and other biological fluids
2.5h
Metabolic pathwayTumor immunityInfection immunityEndocrinologyHematologyHepatology
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken 8-OHdG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.
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