AffiELISA® Bovine Phosphorylated Smad 2 | pSmad2 | ELISA Kit

AffiELISA® Bovine Phosphorylated Smad 2 | pSmad2 | ELISA Kit

The AffiELISA® Bovine Phosphorylated Smad 2 (pSmad2) ELISA Kit is a laboratory tool designed specifically for the quantitative measurement of phosphorylated Smad 2 in bovine samples.

Smad proteins are intracellular signaling molecules that play a crucial role in the transforming growth factor-beta (TGF-β) signaling pathway, a pathway involved in various cellular processes such as cell growth, differentiation, and apoptosis. Smad 2, when phosphorylated (pSmad2), becomes activated and translocates into the cell nucleus, where it regulates the expression of specific target genes involved in these cellular processes.

ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used laboratory technique used to detect and quantify specific proteins or molecules in biological samples. This kit utilizes antibodies that specifically recognize phosphorylated Smad 2 in the bovine samples, enabling the accurate measurement and quantification of its concentration.

By analyzing the levels of phosphorylated Smad 2, researchers can gain insights into the activation status of the TGF-β signaling pathway in bovine samples. This pathway is implicated in various physiological and pathological conditions, including development, tissue repair, immune responses, and diseases such as cancer and fibrosis.

Studying phosphorylated Smad 2 using this ELISA kit facilitates the understanding of its involvement in cellular signaling pathways, providing valuable information for research into its roles in bovine biology, diseases, and potential therapeutic interventions.

Bovine Phosphorylated Smad 2 Background 

Phosphorylated Smad 2 (pSmad2) in bovines refers to the activated form of the Smad 2 protein that has undergone a specific modification through phosphorylation. Smad proteins are a family of intracellular signaling molecules involved in the transforming growth factor-beta (TGF-β) signaling pathway, which regulates various cellular processes.

Smad 2 is part of this signaling pathway, and its activation occurs when it undergoes phosphorylation in response to TGF-β and related signaling molecules. When TGF-β binds to its receptor, it triggers a series of events that result in the phosphorylation of Smad 2. Once phosphorylated, Smad 2 forms complexes with other proteins, translocates to the cell nucleus, and acts as a transcription factor, regulating the expression of specific genes involved in processes like cell growth, differentiation, and responses to injury or stress.

In bovines (cattle), the study of phosphorylated Smad 2 provides insights into the activation status of the TGF-β signaling pathway in various biological contexts. Understanding the activity and regulation of this pathway is crucial for comprehending cellular responses during development, tissue repair, immune function, and disease progression.

Assessing phosphorylated Smad 2 levels through methods like ELISA allows researchers to quantify the extent of pathway activation in bovine samples. This information is valuable for investigating the role of TGF-β signaling in bovine health, reproduction, and potential applications in therapeutic interventions or management practices in cattle-related research.