The ACP (Acid Phosphatase) ELISA Kit is designed for the quantitative measurement of Acid Phosphatase (ACP) levels in biological samples such as serum, plasma, and other fluids. ACP is an enzyme involved in various physiological processes, including bone metabolism and prostate function. It is commonly used as a biomarker for prostate cancer and other conditions.
Content of the Kit
- Pre-coated 96-Well Microplate:
- Wells pre-coated with an antibody specific to ACP, allowing capture of ACP from the samples.
- Standards:
- Solutions containing known concentrations of recombinant or purified ACP, used to generate a standard curve for quantifying ACP levels in samples.
- Sample Diluent:
- A buffer solution used to dilute biological samples to appropriate concentrations for accurate measurement.
- Detection Antibody:
- An antibody specific to a different epitope of ACP, often conjugated to an enzyme such as HRP for detection.
- Enzyme Conjugate:
- If the detection antibody is biotinylated, a streptavidin-HRP conjugate may be included for signal detection.
Procedure
- Coating:
- Coat the wells of the microplate with an antibody specific to ACP. Incubate to allow binding and then wash away unbound antibody.
- Blocking:
- Block any remaining uncoated surfaces in the wells with a blocking buffer to prevent non-specific binding.
- Addition of Standards and Samples:
- Add standards and samples to the wells. The ACP in the samples will bind to the immobilized antibody.
- Incubation:
- Incubate the plate to allow ACP to bind to the antibody.
- Addition of Detection Antibody:
- Add a detection antibody specific to ACP, which binds to the captured ACP.
Performance Characteristics
- Sensitivity: Capable of detecting low levels of ACP, typically in the nanogram per milliliter (ng/mL) range.
- Precision: Shows low intra-assay and inter-assay variability, with coefficients of variation (CV) generally below 10%.
- Linearity: Demonstrates linearity across the standard curve range, ensuring accurate quantification.
- Recovery: High recovery rates, usually above 85%, indicating reliable performance across different sample types.
Applications
- Prostate Health: Used for monitoring prostate health and diagnosing prostate cancer.
- Bone Metabolism: Can be applied in research on bone diseases and conditions affecting bone metabolism.
- Clinical Diagnostics: Assists in clinical diagnosis and monitoring of diseases associated with altered ACP levels.
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