The ADRB (Adrenergic Receptor Beta) ELISA Kit is designed for the quantitative measurement of beta-adrenergic receptors in biological samples such as cell lysates, tissue homogenates, and possibly serum or plasma. Beta-adrenergic receptors (ADRBs) are G protein-coupled receptors that play critical roles in the sympathetic nervous system, regulating heart rate, smooth muscle relaxation, and energy metabolism. These receptors are key targets in cardiovascular research, metabolic studies, and drug development, particularly concerning conditions like hypertension, asthma, and heart failure.
Content of the Kit
- Pre-coated 96-Well Microplate:
- Wells pre-coated with an antibody specific to the ADRB, which captures the receptor protein from the sample.
- Standards:
- Solutions containing known concentrations of recombinant or purified ADRB, used to create a standard curve for quantifying ADRB in samples.
- Sample Diluent:
- A buffer solution used to dilute samples to optimal concentrations for accurate measurement.
- Detection Antibody:
- An antibody specific to a different epitope of the ADRB protein, often conjugated to an enzyme like HRP for detection.
- Streptavidin-HRP Conjugate (if a biotinylated detection antibody is used):
- Binds to the biotinylated detection antibody, enabling colorimetric detection.
- Substrate Solution (TMB):
- Tetramethylbenzidine, which reacts with the HRP enzyme to produce a measurable color change.
Assay Procedure Summary
- Sample and Standard Addition: Samples and standards are added to the microplate wells, where ADRB is captured by the immobilized antibody.
- Wash: Unbound components are removed by washing the wells.
- Detection Antibody Addition: A detection antibody specific to ADRB is added, which binds to the captured receptor.
- Wash: Unbound detection antibody is washed away.
- Enzyme Conjugate Addition: (If applicable) An enzyme-linked conjugate is added, which binds to the detection antibody.
- Wash: Excess enzyme conjugate is removed through washing.
Performance Characteristics
- Sensitivity: Capable of detecting low levels of ADRB, typically in the picogram per milliliter (pg/mL) range.
- Precision: Low intra-assay and inter-assay variability, with coefficients of variation (CV) generally below 10%.
- Linearity: The assay shows a linear response over the concentration range of the standard curve, ensuring accurate quantification across different sample concentrations.
- Recovery: High recovery rates, typically above 85%, ensuring the assay accurately measures ADRB across various sample types.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|