8-OHdG ELISA Kits are specialized enzyme-linked immunosorbent assay (ELISA) kits designed for the quantitative detection of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker of oxidative DNA damage. 8-OHdG is a common marker used in research and clinical diagnostics to assess oxidative stress and DNA damage, which are implicated in various diseases, including cancer, cardiovascular diseases, and neurodegenerative disorders.
Key Features of 8-OHdG ELISA Kits
- High Sensitivity and Specificity:
- Objective: Detect low levels of 8-OHdG with high accuracy.
- Mechanism: Utilize antibodies highly specific to 8-OHdG to minimize cross-reactivity and ensure precise measurement.
- Quantitative Measurement:
- Objective: Provide quantitative data on 8-OHdG levels.
- Mechanism: Generate standard curves from known concentrations of 8-OHdG to accurately quantify the amount in samples.
- Optimized Protocols:
- Procedure: Typically includes coating, blocking, sample incubation, detection, and measurement steps, optimized for efficient and accurate results.
- Time Efficiency: Designed to produce results within a few hours, depending on the protocol specifics.
- Reagent Composition:
- Capture Antibody: Specific for 8-OHdG, immobilized on the microplate.
- Detection Antibody: Conjugated to an enzyme (such as horseradish peroxidase or alkaline phosphatase) that binds to 8-OHdG, producing a detectable signal.
- Substrate Solution: Reacts with the enzyme to produce a measurable colorimetric, fluorometric, or luminescent signal.
- Standard Curve Generation:
- Objective: Calibrate the assay and quantify 8-OHdG levels.
- Mechanism: Include 8-OHdG standards in the assay to create a standard curve for accurate quantification of the target biomarker.
- Sample Types:
- Applicability: Can be used with various sample types, such as serum, plasma, urine, or tissue extracts, depending on the kit specifications.
Applications
- Oxidative Stress Research: Assess oxidative DNA damage and study the impact of oxidative stress on cellular health.
- Disease Diagnosis: Evaluate the role of oxidative damage in diseases such as cancer, cardiovascular diseases, and neurodegenerative disorders.
- Environmental and Lifestyle Studies: Investigate the effects of environmental factors or lifestyle choices on oxidative stress and DNA damage.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|