Aflatoxin ELISA Kits

Aflatoxin ELISA Kits are specialized enzyme-linked immunosorbent assay (ELISA) kits used for the detection and quantification of aflatoxins, which are toxic metabolites produced by certain fungi (e.g., Aspergillus flavus and Aspergillus parasiticus). Aflatoxins are potent carcinogens and can contaminate a variety of food and feed products, making their detection critical for food safety and regulatory compliance.

Key Features of Aflatoxin ELISA Kits

• High Sensitivity and Specificity:
  • Objective: Detect low concentrations of aflatoxins with high accuracy.
  • Mechanism: Utilize antibodies specifically designed to bind aflatoxins, reducing cross-reactivity with other substances and ensuring precise measurement.
• Quantitative Measurement:
  • Objective: Accurately measure the concentration of aflatoxins in samples.
  • Mechanism: Generate standard curves from known concentrations of aflatoxins to quantify their levels in test samples.
• Optimized Protocols:
  • Procedure: Includes steps for coating, blocking, sample incubation, detection, and measurement, all optimized to achieve reliable results.
  • Time Efficiency: Designed to provide results within a few hours, facilitating quick analysis.
• Reagent Composition:
  • Capture Antibody: Specific for aflatoxins, immobilized on a microtiter plate.
  • Detection Antibody: Conjugated to an enzyme (e.g., horseradish peroxidase or alkaline phosphatase) that binds to aflatoxins and produces a detectable signal.
  • Substrate Solution: Reacts with the enzyme to produce a measurable colorimetric, fluorometric, or luminescent signal.

Applications

• Food Safety: Monitor aflatoxin levels in food products to ensure they are within safe limits and comply with regulatory standards.
• Feed Testing: Check for aflatoxin contamination in animal feed to prevent toxic effects on livestock.
• Environmental Monitoring: Assess aflatoxin contamination in agricultural environments to manage and mitigate contamination risks.
• Regulatory Compliance: Meet regulatory requirements for aflatoxin levels in food and feed products.