ATAD ELISA Kits are specialized enzyme-linked immunosorbent assay (ELISA) kits designed to detect and quantify ATPase family AAA domain-containing (ATAD) proteins in various biological samples. ATAD proteins, including ATAD1 and ATAD2, are involved in important cellular processes such as chromatin remodeling, protein degradation, and regulation of gene expression. These kits are crucial for research in cellular and molecular biology, particularly in the context of cancer and other diseases where ATAD proteins play a significant role.

Key Features of ATAD ELISA Kits

• High Sensitivity and Specificity:
  • Objective: Detect low levels of ATAD proteins with high accuracy.
  • Mechanism: Utilize high-affinity antibodies specifically targeting ATAD proteins to ensure precise measurement with minimal cross-reactivity.
• Quantitative Measurement:
  • Objective: Provide accurate quantification of ATAD protein levels in samples.
  • Mechanism: Generate standard curves from known concentrations of ATAD proteins to measure their levels in test samples.
• Optimized Protocols:
  • Procedure: Involves coating, blocking, sample incubation, detection, and measurement steps optimized for reliable and reproducible results.
  • Time Efficiency: Designed to deliver results within a few hours, depending on the specific kit and protocol used.
• Reagent Composition:
  • Capture Antibody: Specific for ATAD proteins, immobilized on the microtiter plate.
  • Detection Antibody: Conjugated to an enzyme (e.g., horseradish peroxidase or alkaline phosphatase) that binds to ATAD proteins and produces a detectable signal.
  • Substrate Solution: Reacts with the enzyme to produce a measurable signal (colorimetric, fluorometric, or luminescent).
• Standard Curve Generation:
  • Objective: Calibrate the assay for accurate quantification.
  • Mechanism: Include ATAD protein standards in the assay to create a standard curve for determining the concentration of ATAD proteins in the samples.
• Sample Types:
  • Applicability: Suitable for various sample types, including cell lysates, tissue extracts, serum, and plasma, depending on the kit specifications.

Applications

• Cancer Research: Study the role of ATAD proteins in tumorigenesis, cell proliferation, and apoptosis.
• Cellular Biology: Investigate the involvement of ATAD proteins in chromatin remodeling, gene expression regulation, and protein degradation.
• Genetic Research: Explore the effects of mutations or altered expression of ATAD genes on cellular function and disease.
• Drug Development: Assess the impact of therapeutic agents targeting ATAD proteins in preclinical and clinical studies.
• Diagnostics: Aid in the early detection and monitoring of diseases associated with dysregulated ATAD protein levels.