ATAD ELISA Kits

ATAD (ATPase Family AAA Domain Containing) ELISA Kits are immunoassay tools specifically developed to quantify the levels of ATAD proteins, which play roles in various cellular processes including protein degradation, cellular signaling, and organelle biogenesis. These kits primarily target ATAD1 and ATAD3 isoforms, which are of particular interest in studies of mitochondrial dynamics and neurodegenerative disease mechanisms. Using highly specific antibodies against ATAD proteins, these kits provide reliable quantification from biological samples, such as cell lysates, serum, and tissue extracts.

Content

ATAD ELISA Kits typically include:

• Pre-coated microplates with capture antibodies specific to ATAD proteins.
• Detection antibodies conjugated to enzymes, typically horseradish peroxidase (HRP), allowing for quantification via chromogenic or fluorometric readouts.
• Standards and controls to ensure quantifiable results across different samples and facilitate comparison.
• Buffers and reagents for assay optimization, including wash, blocking, and substrate solutions to enhance assay specificity and minimize background noise.

The assay involves sample binding to capture antibodies on the plate, followed by detection with a secondary enzyme-labeled antibody, generating a signal proportional to the ATAD concentration in the sample.

Applications

ATAD ELISA Kits are utilized in:

• Mitochondrial function research: Quantifying ATAD levels to study mitochondrial biogenesis, dynamics, and quality control.
• Neurodegenerative disease research: Investigating the role of ATAD proteins in neurodegeneration, as disruptions in ATPase function can lead to protein aggregation and cellular stress.
• Cancer research: Analyzing ATAD expression in tumor cells, where ATAD proteins may influence proliferation and cell survival by regulating proteostasis and organelle maintenance.

ATAD ELISA Kits offer a standardized method for assessing ATAD protein levels, supporting research into mitochondrial regulation, cellular energy dynamics, and potential therapeutic approaches targeting ATPase-related pathways.