AffiELISA® CAMP ELISA Kits

Overview of CAMP ELISA Kits

CAMP (Cyclic Adenosine Monophosphate) ELISA kits are laboratory tools used for the detection and quantification of cAMP in various biological samples such as plasma, serum, urine, cell lysates, and tissue homogenates. cAMP is a second messenger important in many biological processes, including the regulation of glycogen, sugar, and lipid metabolism. The detection of cAMP is crucial in research areas such as cell signaling, endocrinology, and pharmacology.

Content of CAMP ELISA Kits

ELISA Plate: Pre-coated with a specific antibody that captures cAMP from samples.
cAMP Standard: A known concentration of cAMP to create a standard curve for quantification.
Binding Reagent: Used to bind cAMP to the antibody-coated plate.
HRP-Conjugated cAMP: A cAMP molecule linked to an enzyme (horse radish peroxidase, HRP) for detection.
Substrate Solution: Reacts with HRP to produce a measurable color change.
Stop Solution: Stops the enzyme-substrate reaction to fix the color for measurement.
Wash Buffer: Used to wash away unbound substances between steps.
Assay Buffer: Provides optimal conditions for the binding reactions.
Plate Seals: Prevents contamination and evaporation during incubation.

Procedure

Sample Preparation: Prepare samples and standards.
Coating: Add samples and standards to the ELISA plate wells.
Binding: Add binding reagent to capture cAMP.
Detection: Add HRP-conjugated cAMP to compete with sample cAMP for antibody binding.
Washing: Wash the plate to remove unbound components.
Substrate Addition: Add the substrate solution to the wells.
Reaction Stop: Add the stop solution once the desired color change is achieved.
Measurement: Measure the absorbance using a plate reader at the specified wavelength.

Key Considerations

Sensitivity and Specificity: Ensure the kit is sensitive enough to detect the expected range of cAMP concentrations.
Sample Handling: Proper handling and preparation of samples are critical to obtain accurate results.
Standard Curve: Generate a standard curve with each assay to ensure accurate quantification.
Incubation Times: Follow recommended incubation times strictly to ensure optimal reactions.