DDX ELISA Kits are designed for the quantitative measurement of DEAD-box (DDX) proteins in various biological samples. DDX proteins are a family of RNA helicases involved in numerous aspects of RNA metabolism, including transcription, splicing, translation, and ribosome biogenesis.

Technical Content

Key Features:

• High Sensitivity: Capable of detecting low levels of DDX proteins, ensuring precise quantification even in samples with low expression.
• Specificity: High specificity for DDX proteins, minimizing cross-reactivity with other proteins.
• Wide Range of Applications: Suitable for use with serum, plasma, cell lysates, and other biological fluids.

Applications:

• Molecular Biology Research: Used to study the role of DDX proteins in RNA metabolism and regulation.
• Cancer Research: Facilitates the investigation of DDX proteins' roles in tumorigenesis, cancer progression, and response to therapy.
• Gene Expression Studies: Assists in understanding the involvement of DDX proteins in gene expression regulation and RNA processing.

Usage Protocol:

• Sample Preparation: Collect and prepare samples according to the kit instructions. Dilute if necessary.
• Assay Procedure: Add samples, standards, and controls to the wells of the ELISA plate pre-coated with DDX-specific antibodies.
• Incubation: Incubate the plate to allow binding of DDX proteins to the antibodies.
• Detection: Add detection antibodies and enzyme substrate to generate a colorimetric signal proportional to the DDX protein concentration.
• Analysis: Measure the absorbance using a microplate reader and calculate DDX protein concentrations based on the standard curve.

Benefits:

• Precision: Provides reliable and reproducible results for the accurate quantification of DDX proteins.
• Efficiency: Simplified protocol with ready-to-use reagents reduces assay time and labor.
• Versatility: Compatible with a variety of sample types, making it suitable for diverse research applications.