DDX ELISA Kits

DDX (DEAD-box RNA helicase) ELISA Kits are designed to detect and quantify proteins in the DDX family, such as DDX3, DDX4, and DDX5, which play crucial roles in RNA metabolism, including RNA splicing, translation initiation, and ribosome assembly. DDX proteins are characterized by their helicase activity, aiding in unwinding RNA structures. They are central to cellular processes like gene expression regulation and stress response and are being investigated in cancer, viral infections, and neurodegenerative diseases. DDX ELISA Kits provide a sensitive, accurate means for quantifying these proteins in biological samples, such as cell lysates and tissue extracts.

Content

DDX ELISA Kits typically contain:

• Microplates pre-coated with capture antibodies specific to DDX proteins, targeting distinct DDX isoforms.
• Detection antibodies, often enzyme-conjugated (e.g., with horseradish peroxidase), which generate a signal when bound to the captured DDX proteins.
• Standards and controls to create a standard curve, allowing precise quantification of DDX levels across samples.
• Buffers and reagents, including wash, blocking, and substrate solutions, which optimize assay sensitivity and reduce background noise.

The assay procedure involves binding DDX proteins in the sample to capture antibodies on the plate, followed by detection with an enzyme-conjugated antibody. The resulting enzyme-substrate reaction yields a signal proportional to the DDX protein concentration in the sample.

Applications

DDX ELISA Kits are used in:

• Cancer research: Studying DDX proteins involved in cell proliferation and survival pathways, as several DDX proteins are upregulated in cancer.
• Viral infection studies: Investigating DDX proteins’ roles in viral replication, as many viruses hijack host helicases like DDX to facilitate their replication.
• Neurodegenerative disease research: Examining DDX proteins in neurodegenerative conditions where RNA metabolism is disrupted.
• Gene expression studies: Quantifying DDX proteins to understand RNA processing and regulation in various cellular conditions.

DDX ELISA Kits support high-specificity and reproducible quantification of DDX proteins, advancing research on RNA helicases in disease mechanisms and potential therapeutic targets.