DHT ELISA Kits

DHT (Dihydrotestosterone) ELISA Kits are immunoassays designed to quantify levels of dihydrotestosterone, an androgen hormone derived from testosterone, in biological samples such as serum, plasma, and tissue extracts. DHT plays a vital role in male sexual development and is implicated in conditions such as androgenic alopecia, prostate disorders, and polycystic ovary syndrome (PCOS). Due to its significance in both normal physiology and pathology, DHT measurement is crucial in endocrinology, reproductive health, and clinical research.

Content

DHT ELISA Kits typically contain:

• Microplates pre-coated with capture antibodies that specifically bind DHT.
• Enzyme-conjugated DHT or detection antibodies, which compete with sample DHT for binding sites on the antibodies, facilitating detection via a competitive binding format.
• Standards and controls to establish a standard curve, allowing accurate DHT quantification across samples.
• Buffers and reagents, including wash, blocking, and substrate solutions, to enhance specificity and minimize background noise.

The assay involves a competitive binding mechanism, where sample DHT competes with enzyme-conjugated DHT for limited antibody binding sites on the plate. Signal intensity is inversely proportional to the DHT concentration, as higher sample DHT levels reduce the binding of enzyme-labeled DHT, leading to lower signal output.

Applications

DHT ELISA Kits are widely used in:

• Endocrinology research: Measuring DHT levels to understand androgen regulation, male sexual development, and hormonal balance in both sexes.
• Androgenic alopecia studies: Monitoring DHT in individuals with hair loss, as DHT is closely linked to androgenic alopecia (male and female pattern baldness).
• Prostate health research: Quantifying DHT to investigate its role in benign prostatic hyperplasia (BPH) and prostate cancer.
• PCOS and reproductive health: Assessing DHT in conditions like PCOS, where elevated androgen levels can disrupt reproductive function.

DHT ELISA Kits provide a reliable, standardized method for DHT measurement, supporting research into hormonal pathways, endocrine disorders, and potential treatments targeting androgen-related conditions.