FBG ELISA Kits (Fibrinogen ELISA Kits) are specialized enzyme-linked immunosorbent assay kits designed to detect and quantify fibrinogen levels in biological samples. Fibrinogen is a crucial plasma protein involved in blood clot formation and is an important biomarker for assessing coagulation status, inflammation, and cardiovascular risk.

Key Features of FBG ELISA Kits

• High Sensitivity and Specificity:
  • Objective: Detect low concentrations of fibrinogen with high accuracy.
  • Mechanism: Utilize high-affinity antibodies specifically targeting fibrinogen to ensure precise measurement with minimal cross-reactivity.
• Quantitative Measurement:
  • Objective: Provide accurate quantification of fibrinogen levels in samples.
  • Mechanism: Generate standard curves from known concentrations of fibrinogen to measure its levels in test samples.
• Optimized Protocols:
  • Procedure: Includes coating, blocking, sample incubation, detection, and measurement steps optimized for reliable and reproducible results.
  • Time Efficiency: Designed to deliver results within a few hours, depending on the specific kit and protocol used.
• Reagent Composition:
  • Capture Antibody: Specific for fibrinogen, immobilized on the microtiter plate.
  • Detection Antibody: Conjugated to an enzyme (e.g., horseradish peroxidase or alkaline phosphatase) that binds to fibrinogen and produces a detectable signal.
  • Substrate Solution: Reacts with the enzyme to produce a measurable signal (colorimetric, fluorometric, or luminescent).
• Standard Curve Generation:
  • Objective: Calibrate the assay for accurate quantification.
  • Mechanism: Include fibrinogen standards in the assay to create a standard curve for determining the concentration of fibrinogen in the samples.
• Sample Types:
  • Applicability: Suitable for various sample types, including serum, plasma, and other body fluids, depending on the kit specifications.

Applications

• Coagulation Disorders: Assess fibrinogen levels to diagnose and monitor disorders related to abnormal blood clotting, such as hemophilia and disseminated intravascular coagulation (DIC).
• Inflammation and Infection: Monitor fibrinogen levels as an inflammatory marker in conditions such as rheumatoid arthritis and sepsis.
• Cardiovascular Risk Assessment: Evaluate fibrinogen levels as a risk factor for cardiovascular diseases, including stroke and myocardial infarction.
• Surgical and Trauma Monitoring: Measure fibrinogen levels to manage bleeding risk and guide treatment during and after surgery or trauma.
• Research: Study fibrinogen dynamics in various biological and pathological processes.