Overview of IL6 ELISA Kits
IL6 ELISA Kits are designed for the quantitative measurement of Interleukin-6 (IL-6) levels in various biological samples. IL-6 is a cytokine involved in inflammation and the immune response, playing a critical role in various physiological and pathological processes.
Technical Content
Key Features:
- High Sensitivity: Capable of detecting low levels of IL-6 in samples, ensuring accurate measurement even in cases of low cytokine expression.
- Specificity: High specificity for IL-6, minimizing cross-reactivity with other cytokines and proteins.
- Wide Range of Applications: Suitable for use with serum, plasma, cell culture supernatants, and other biological fluids.
Applications:
- Clinical Research: Used to study inflammatory diseases, autoimmune disorders, and cancer by measuring IL-6 levels in patient samples.
- Immunology Studies: Facilitates the investigation of immune responses and cytokine signaling pathways.
- Drug Development: Assists in evaluating the efficacy of anti-inflammatory and immunomodulatory drugs by monitoring IL-6 levels.
Usage Protocol:
- Sample Preparation: Collect and prepare samples according to the kit instructions. Dilute if necessary.
- Assay Procedure: Add samples, standards, and controls to the wells of the ELISA plate pre-coated with IL-6 antibodies.
- Incubation: Incubate the plate to allow IL-6 to bind to the antibodies.
- Detection: Add detection antibodies and enzyme substrate to generate a colorimetric signal proportional to the IL-6 concentration.
- Analysis: Measure the absorbance using a microplate reader and calculate IL-6 concentrations based on the standard curve.
Benefits:
- Accuracy: Provides reliable and reproducible results for quantitative analysis of IL-6.
- Efficiency: Streamlined protocol with ready-to-use reagents reduces assay time and labor.
- Versatility: Compatible with a variety of sample types, making it suitable for diverse research needs.
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