KLH ELISA Kits

KLH (Keyhole Limpet Hemocyanin) ELISA kits are immunoassays designed for the quantitative detection of anti-KLH antibodies or KLH antigens in biological samples such as serum or plasma. KLH is a large, oxygen-carrying protein derived from the marine mollusk Megathura crenulata and is commonly used as a carrier protein for haptens and as an immune stimulant in vaccine research. These kits are critical for assessing immune responses in preclinical and clinical studies involving KLH as a model antigen.

Content of KLH ELISA Kits: KLH ELISA kits typically include:

• A 96-well microplate pre-coated with KLH or anti-KLH antibodies.
• Calibrated KLH standards for constructing a standard curve.
• Secondary detection antibodies, which may be enzyme-conjugated (e.g., HRP).
• Substrate solution such as TMB (3,3',5,5'-Tetramethylbenzidine) for colorimetric signal development.
• Stop solution to halt the enzymatic reaction.
• Assay buffers, wash buffers, and sample diluents.
• Detailed protocol instructions for preparing and running the assay.

Application of KLH ELISA Kits: KLH ELISA kits are used in various research and diagnostic applications, including:

• Immunogenicity testing: Measuring anti-KLH antibody titers in samples to evaluate immune responses, particularly in vaccine and immunotherapy studies.
• Vaccine development: Assessing the efficacy of KLH-conjugated vaccines by quantifying the immune response generated against the hapten-KLH complex.
• Immunotherapy research: Using KLH as an adjuvant in cancer immunotherapy research to enhance the immune system's ability to recognize and fight tumor cells.
• Preclinical and clinical trials: Monitoring immune responses in patients or animal models that have been administered KLH-based vaccines or treatments.
• Basic immunology studies: Employing KLH as a model antigen to investigate immune mechanisms and pathways, aiding in the understanding of immune system dynamics and antigen processing.

KLH ELISA kits provide precise, reproducible data that are essential for the evaluation of immune responses, making them valuable tools in immunology research, vaccine development, and clinical studies.