Lactoferrin ELISA Kits

Lactoferrin ELISA kits are immunoassays designed for the quantitative detection of lactoferrin in various biological samples such as feces, serum, plasma, and milk. Lactoferrin is an iron-binding glycoprotein found in bodily fluids and is a key component of the immune system, with antimicrobial and anti-inflammatory properties. The use of lactoferrin ELISA kits is essential in clinical and research settings for studying gastrointestinal disorders, infections, and inflammation.

Content of Lactoferrin ELISA Kits: Lactoferrin ELISA kits generally contain:

• A 96-well microplate pre-coated with anti-lactoferrin antibodies.
• Recombinant lactoferrin standards for calibration curve generation.
• Enzyme-conjugated detection antibodies, typically linked to HRP (Horseradish Peroxidase).
• Substrate solution such as TMB (3,3',5,5'-Tetramethylbenzidine) for colorimetric signal development.
• Stop solution to halt the enzymatic reaction.
• Sample diluents, wash buffers, and assay buffers.
• An instruction manual detailing protocols for sample preparation and assay procedures.

Application of Lactoferrin ELISA Kits: Lactoferrin ELISA kits are used for multiple research and clinical purposes, including:

• Diagnosis of gastrointestinal inflammation: Measuring fecal lactoferrin as a non-invasive biomarker to detect inflammation associated with conditions like inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis.
• Infection monitoring: Assessing lactoferrin levels as an indicator of bacterial infection due to its release by activated neutrophils during infection and inflammation.
• Nutritional studies: Investigating lactoferrin concentrations in breast milk and its role in infant nutrition and immune development.
• Immunology research: Understanding the role of lactoferrin in modulating immune responses, as it exhibits both antimicrobial and immunoregulatory activities.
• Clinical research: Evaluating lactoferrin as a potential biomarker for systemic inflammatory conditions and studying its therapeutic potential due to its natural antimicrobial and anti-inflammatory properties.

Lactoferrin ELISA kits provide reliable, quantitative results crucial for understanding the presence and function of lactoferrin in biological samples, supporting research and diagnostics related to inflammation, infection, and immune health.