MTHFD ELISA Kits

MTHFD (Methylenetetrahydrofolate Dehydrogenase) ELISA kits are specialized immunoassays designed for the quantitative detection of MTHFD proteins in biological samples such as cell lysates, serum, and tissue extracts. MTHFD is a multifunctional enzyme that plays a critical role in one-carbon metabolism, participating in folate-mediated biochemical pathways essential for DNA synthesis, repair, and cellular methylation processes. MTHFD is particularly significant in studies related to cancer, metabolic disorders, and cellular growth regulation.

Content of MTHFD ELISA Kits: MTHFD ELISA kits typically include:

• A 96-well microplate pre-coated with capture antibodies specific to MTHFD proteins.
• Recombinant MTHFD standards for constructing a standard curve.
• Detection antibodies conjugated to an enzyme (e.g., HRP – Horseradish Peroxidase) for signal generation.
• Substrate solution (e.g., TMB – 3,3',5,5'-Tetramethylbenzidine) for color development.
• Stop solution to terminate the enzymatic reaction.
• Sample diluents, wash buffers, and assay buffers.
• Detailed instructions for sample preparation and assay procedure.

Application of MTHFD ELISA Kits: MTHFD ELISA kits are used in various research and clinical applications, including:

• Cellular growth and differentiation: Exploring the impact of MTHFD on cell growth and differentiation, given its role in providing one-carbon units for nucleotide synthesis and methylation reactions.
• Drug development: Screening potential inhibitors of MTHFD for therapeutic interventions aimed at disrupting one-carbon metabolism in cancer cells or addressing metabolic disorders.
• Nutritional and folate research: Studying the relationship between MTHFD activity and folate intake or supplementation to understand how it affects metabolic health and disease risk.
• Molecular biology: Analyzing MTHFD’s role in DNA repair and synthesis processes, as these functions are critical for maintaining genomic stability.

MTHFD ELISA kits are invaluable for researchers studying folate metabolism, cancer biology, and related areas, providing reliable and precise data for understanding the complex roles of MTHFD in health and disease.