MTHFD (Methylenetetrahydrofolate dehydrogenase) ELISA kits are designed to measure the levels of MTHFD, an enzyme involved in folate metabolism and one-carbon metabolism. Accurate measurement of MTHFD is important for research into metabolic disorders and conditions related to folate metabolism.
Components
- Coated Microplate:
- A 96-well plate pre-coated with antibodies specific to MTHFD. This allows MTHFD in the sample to bind to the plate.
- Standards:
- Recombinant or synthetic MTHFD proteins with known concentrations used to create a standard curve for quantification.
- Detection Antibody:
- A secondary antibody specific to MTHFD, typically conjugated to an enzyme such as horseradish peroxidase (HRP) for detection.
- Substrate Solution:
- Contains a substrate that reacts with the HRP enzyme to produce a color change proportional to the amount of MTHFD present.
- Stop Solution:
- An acid solution that halts the enzyme reaction and stabilizes the color for measurement.
- Wash Buffer:
- Used to wash away unbound substances and reduce background noise, ensuring only specifically bound MTHFD is measured
Technical Considerations
- Sensitivity:
- The minimum detectable concentration of MTHFD.
- Specificity:
- Ability to specifically detect MTHFD without cross-reacting with other proteins.
- Reproducibility:
- Consistency of assay results across different tests and conditions.
- Interferences:
- Potential effects of other substances in the sample on assay accuracy.
Applications
- Research: Studying MTHFD's role in folate metabolism and related metabolic processes.
- Clinical Diagnostics: Assessing MTHFD levels in conditions related to folate metabolism or deficiencies.
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