NuMA ELISA Kits

NuMA (Nuclear Mitotic Apparatus protein) ELISA kits are immunoassays designed for the quantitative detection of NuMA in biological samples such as cell lysates and tissue extracts. NuMA is a structural protein that plays a crucial role in the organization and stability of the mitotic spindle during cell division. It is essential for proper chromosome segregation and cell cycle progression. The study of NuMA is particularly relevant in cell biology, cancer research, and studies related to cell proliferation and differentiation.

Content of NuMA ELISA Kits: NuMA ELISA kits typically include:

• A 96-well microplate pre-coated with capture antibodies specific to NuMA.
• Recombinant NuMA protein standards for the creation of a standard curve.
• Detection antibodies, often conjugated to an enzyme such as HRP (Horseradish Peroxidase) for signal amplification.
• Substrate solution, typically TMB (3,3',5,5'-Tetramethylbenzidine), for colorimetric detection.
• Stop solution to terminate the enzymatic reaction.
• Sample diluents, assay buffers, and wash buffers.
• A comprehensive protocol for assay preparation and sample handling.

Application of NuMA ELISA Kits: NuMA ELISA kits are used in various research contexts, including:

• Cell cycle and mitosis research: Quantifying NuMA levels to investigate its role in mitotic spindle formation, chromosome alignment, and overall cell cycle progression.
• Cancer research: Studying NuMA's involvement in tumorigenesis and its potential as a biomarker, as alterations in mitotic processes can contribute to aneuploidy and cancer development.
• Cell proliferation and differentiation studies: Evaluating NuMA's function in different cell types and its role in cellular organization, especially during differentiation processes.
• Apoptosis and cell death research: Investigating the potential interaction of NuMA with pathways involved in programmed cell death, particularly where cell division errors may trigger apoptotic mechanisms.
• Drug discovery and development: Screening for compounds that modulate NuMA expression or function, especially in the context of cancer therapy, to disrupt aberrant cell division.
• Genetic and developmental biology: Analyzing NuMA's expression patterns during various stages of cell and tissue development.

NuMA ELISA kits provide sensitive, accurate quantification of NuMA, supporting research into cellular mechanisms underlying division, structural organization, and disease progression.