PADI ELISA Kits

PADI (Peptidyl Arginine Deiminase) ELISA kits are immunoassays designed for the quantitative detection of PADI enzymes in biological samples such as serum, plasma, and cell lysates. PADIs are a family of enzymes that catalyze the post-translational conversion of arginine residues into citrulline, a process known as citrullination or deimination. 

Content of PADI ELISA Kits: PADI ELISA kits typically include:

• A 96-well microplate pre-coated with capture antibodies specific for PADI isoforms (e.g., PADI2, PADI4).
• Recombinant PADI protein standards for generating a standard curve.
• Detection antibodies, often conjugated to an enzyme such as HRP (Horseradish Peroxidase).
• Substrate solution (commonly TMB – 3,3',5,5'-Tetramethylbenzidine) for colorimetric detection.
• Stop solution to terminate the enzymatic reaction.
• Assay buffers, wash buffers, sample diluents, and detailed instructions for performing the assay.

Application of PADI ELISA Kits: PADI ELISA kits are used for various research and clinical applications, including:

• Autoimmune disease research: Measuring PADI expression and activity to study its role in the pathogenesis of autoimmune conditions like rheumatoid arthritis, where the enzyme contributes to the formation of anti-citrullinated protein antibodies (ACPAs).
• Inflammatory research: Investigating the involvement of PADIs in inflammation and their regulation under different inflammatory stimuli.
• Cancer research: Studying PADI's role in tumor biology, as citrullination by PADI enzymes can influence cancer progression, immune evasion, and metastasis.
• Neurodegenerative diseases: Exploring PADI’s contribution to diseases like multiple sclerosis, where aberrant citrullination may affect myelin integrity and immune responses in the central nervous system.
• Cellular and molecular biology: Analyzing PADI expression in various cell types to understand its regulatory effects on gene expression and protein function through citrullination.
• Drug development: Screening potential inhibitors of PADI enzymes as therapeutic candidates for autoimmune and inflammatory diseases.

PADI ELISA kits provide reliable, quantitative data crucial for elucidating the roles of PADI enzymes in health and disease, aiding in the development of new therapeutic strategies and advancing the understanding of citrullination in biological processes.