PCT ELISA Kits

PCT (Procalcitonin) ELISA kits are immunoassays designed for the quantitative detection of procalcitonin levels in biological samples such as serum and plasma. Procalcitonin is a precursor of the hormone calcitonin and is produced by various tissues in response to bacterial infections. It serves as an important biomarker for systemic inflammation and sepsis, with elevated levels indicating severe bacterial infections. PCT ELISA kits are essential tools in clinical diagnostics and research focused on infectious diseases and sepsis management.

Content of PCT ELISA Kits: PCT ELISA kits typically include:

• A 96-well microplate pre-coated with capture antibodies specific to procalcitonin.
• Recombinant procalcitonin standards for the construction of a calibration curve.
• Detection antibodies, often enzyme-conjugated (e.g., HRP) to facilitate signal generation.
• Substrate solution, commonly TMB (3,3',5,5'-Tetramethylbenzidine), for colorimetric detection.
• Stop solution to halt the enzymatic reaction.
• Assay diluents, wash buffers, and sample diluents.
• A detailed instruction manual for sample handling and assay procedures.

Application of PCT ELISA Kits: PCT ELISA kits have various important applications, including:

• Sepsis diagnosis and monitoring: Measuring procalcitonin levels to aid in the early detection and monitoring of sepsis, allowing for timely and targeted treatment decisions.
• Differentiating bacterial from viral infections: Using procalcitonin as a biomarker to distinguish between bacterial and non-bacterial infections, as PCT levels typically remain low in viral infections.
• Critical care and emergency medicine: Assessing PCT levels in critically ill patients to evaluate the severity of systemic bacterial infections and guide antibiotic therapy.
• Post-surgical infection detection: Monitoring PCT levels to identify potential bacterial infections following surgical procedures.
• Inflammatory disease research: Investigating the role of procalcitonin in the context of various inflammatory conditions and its regulation in different disease states.
• Pharmaceutical development: Evaluating the efficacy of new antimicrobial and anti-inflammatory drugs by monitoring changes in procalcitonin levels in clinical or preclinical studies.

PCT ELISA kits provide high sensitivity and specificity for procalcitonin detection, making them valuable tools for clinical diagnostics and research aimed at understanding and managing bacterial infections and sepsis.