Plasminogen Activator Inhibitor ELISA Kits

Plasminogen Activator Inhibitor (PAI) ELISA kits are immunoassays used for the quantitative measurement of PAI proteins, primarily PAI-1 and PAI-2, in biological samples such as plasma, serum, and cell culture supernatants. PAI-1 is a key regulator of fibrinolysis, acting as the primary inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA). It plays a significant role in coagulation, thrombosis, and various pathological conditions such as cardiovascular diseases, cancer, and metabolic disorders. PAI-2 is involved in cellular processes and inflammatory responses.

Content of PAI ELISA Kits: PAI ELISA kits typically contain:

• A 96-well microplate pre-coated with capture antibodies specific to PAI-1 or PAI-2.
• Recombinant PAI standards for calibration curve generation.
• Detection antibodies, often conjugated to an enzyme like HRP (Horseradish Peroxidase) for colorimetric signal detection.
• Substrate solution (commonly TMB – 3,3',5,5'-Tetramethylbenzidine) for signal development.
• Stop solution to halt the enzymatic reaction.
• Sample and assay diluents, wash buffers, and comprehensive assay instructions.

Application of PAI ELISA Kits: PAI ELISA kits are utilized in various research and clinical applications, including:

• Cardiovascular disease research: Measuring PAI-1 levels to study its role in thrombosis and impaired fibrinolysis, as elevated PAI-1 is associated with an increased risk of cardiovascular events such as myocardial infarction and stroke.
• Metabolic syndrome and diabetes: Investigating the involvement of PAI-1 in metabolic disorders, as it is often elevated in conditions like obesity, insulin resistance, and type 2 diabetes.
• Cancer research: Analyzing PAI expression, especially PAI-1, which can promote tumor progression, invasion, and metastasis by regulating the balance of proteolytic activity in the extracellular matrix.
• Wound healing and tissue repair: Studying the role of PAI proteins in tissue remodeling and the regulation of fibrinolysis during the wound healing process.
• Inflammatory response studies: Evaluating the contribution of PAI-2 to immune and inflammatory responses in various diseases.
• Drug development: Assessing the effects of potential therapeutic agents targeting PAI pathways for diseases where abnormal fibrinolytic regulation plays a part.

PAI ELISA kits provide reliable, quantitative analysis for research into hemostasis, vascular diseases, and cancer, offering high specificity and sensitivity to support experimental and clinical investigations.