PRL ELISA Kits

PRL (Prolactin) ELISA kits are immunoassays designed for the quantitative detection of prolactin levels in biological samples such as serum, plasma, and cell culture supernatants. Prolactin is a peptide hormone primarily produced by the anterior pituitary gland, playing crucial roles in lactation, reproductive health, and metabolic processes. Its measurement is vital in research and clinical diagnostics for conditions involving reproductive health, endocrine disorders, and certain pituitary tumors.

Content of PRL ELISA Kits: PRL ELISA kits typically include:

• A 96-well microplate pre-coated with capture antibodies specific for prolactin.
• Recombinant prolactin standards for generating a standard curve.
• Enzyme-conjugated detection antibodies (e.g., HRP) for enhanced signal detection.
• Substrate solution (typically TMB – 3,3',5,5'-Tetramethylbenzidine) for colorimetric readout.
• Stop solution to terminate the enzymatic reaction.
• Sample and assay diluents, wash buffers, and detailed instructions for assay procedures and sample preparation.

Application of PRL ELISA Kits: PRL ELISA kits are used in various research and clinical applications, including:

• Endocrine disorder diagnosis: Measuring prolactin levels to help diagnose and monitor conditions such as hyperprolactinemia, which can be associated with pituitary adenomas or hypothyroidism.
• Reproductive health studies: Assessing prolactin in the context of reproductive health, fertility, and pregnancy-related investigations, as abnormal prolactin levels can affect menstrual cycles and fertility.
• Pituitary function evaluation: Evaluating prolactin secretion as part of overall pituitary function testing, aiding in the detection of pituitary gland abnormalities.
• Metabolic and stress-related research: Investigating the role of prolactin in metabolic regulation, stress response, and immune modulation.
• Drug efficacy and safety studies: Monitoring changes in prolactin levels as a response to certain medications, such as antipsychotic drugs that may induce hyperprolactinemia.
• Breastfeeding and lactation studies: Analyzing prolactin as a critical hormone in lactation to understand its regulation and effects on milk production.

PRL ELISA kits provide high specificity and sensitivity for accurate quantification, making them essential tools in both research and clinical diagnostics focused on endocrine and reproductive health.