SIRT ELISA Kits

SIRT (Sirtuin) ELISA kits are immunoassays designed for the quantitative detection of Sirtuin proteins in biological samples such as cell lysates, serum, plasma, and tissue extracts. Sirtuins are a family of NAD+-dependent deacetylases that play crucial roles in cellular regulation, including aging, metabolism, DNA repair, and stress resistance. These kits are valuable tools in research focused on aging, metabolic disorders, neurodegenerative diseases, and cancer, where Sirtuin activity is a significant factor.

Content of SIRT ELISA Kits: SIRT ELISA kits typically include:

• A 96-well microplate pre-coated with specific capture antibodies targeting Sirtuin isoforms (e.g., SIRT1, SIRT2, etc.).
• Recombinant Sirtuin standards for creating a standard curve.
• Detection antibodies, often conjugated with an enzyme such as HRP (Horseradish Peroxidase), for signal development.
• Substrate solution (e.g., TMB – 3,3',5,5'-Tetramethylbenzidine) for colorimetric detection.
• Stop solution to halt the enzymatic reaction.
• Sample diluents, wash buffers, assay buffers, and a detailed protocol for assay execution and sample preparation.

Application of SIRT ELISA Kits: SIRT ELISA kits are used in a variety of research and clinical applications, including:

• Aging research: Quantifying Sirtuin levels to study their role in the regulation of aging processes and age-related diseases, such as cardiovascular and neurodegenerative disorders.
• Metabolic studies: Assessing Sirtuins' involvement in metabolic regulation, particularly in conditions such as obesity, type 2 diabetes, and metabolic syndrome, as they influence pathways like insulin sensitivity and lipid metabolism.
• Cancer research: Investigating the role of different Sirtuin isoforms in tumorigenesis, where they can act as both tumor suppressors and promoters, depending on the context.
• Neurodegenerative disease research: Studying Sirtuins for their neuroprotective properties and their potential role in diseases like Alzheimer’s and Parkinson’s.
• Cellular stress and DNA repair: Exploring the function of Sirtuins in cellular response to oxidative stress and DNA damage, which contributes to maintaining genomic stability.
• Drug development: Screening and evaluating compounds that modulate Sirtuin activity, which could lead to potential therapies for metabolic disorders, neurodegeneration, and other Sirtuin-related diseases.

SIRT ELISA kits provide sensitive and specific quantification, making them essential for researchers investigating the complex roles of Sirtuins in health and disease.