SMC ELISA Kits

SMC (Structural Maintenance of Chromosomes) ELISA kits are immunoassays designed for the quantitative detection of SMC proteins in biological samples such as cell lysates and tissue extracts. SMC proteins play essential roles in chromosome organization, cohesion, and segregation during cell division. They form the core components of multi-protein complexes like cohesin and condensin, which are critical for maintaining genome stability and proper chromosome dynamics. 

Content of SMC ELISA Kits: SMC ELISA kits typically include:

• A 96-well microplate pre-coated with capture antibodies specific to SMC proteins (e.g., SMC1, SMC2, SMC3).
• Recombinant SMC protein standards for constructing a standard curve.
• Detection antibodies conjugated to enzymes such as HRP (Horseradish Peroxidase) for signal detection.
• Substrate solution (e.g., TMB – 3,3',5,5'-Tetramethylbenzidine) for colorimetric readout.
• Stop solution to terminate the enzymatic reaction.
• Sample diluents, wash buffers, assay buffers, and detailed protocols for sample handling and assay procedures.

Application of SMC ELISA Kits: SMC ELISA kits are used in various research and clinical applications, including:

• Cell cycle and mitosis research: Quantifying SMC protein levels to study their role in chromosomal cohesion and condensation, essential for accurate segregation of chromosomes during cell division.
• Cancer research: Investigating the involvement of SMC proteins in tumorigenesis, as disruptions in SMC function can lead to chromosomal instability and contribute to cancer development and progression.
• Genomic stability studies: Exploring the role of SMC proteins in DNA repair mechanisms and maintaining the structural integrity of chromosomes.
• Developmental biology: Analyzing SMC protein function in the context of cell differentiation and organismal development.

SMC ELISA kits provide precise and reproducible quantification, making them essential tools for researchers studying chromosome dynamics, genome maintenance, and associated diseases.