Synuclein ELISA Kits

Synuclein ELISA kits are immunoassays designed for the quantitative detection of synuclein proteins, such as alpha-synuclein, beta-synuclein, and gamma-synuclein, in biological samples like cerebrospinal fluid (CSF), serum, plasma, and tissue lysates. Synucleins are a family of neuronal proteins that play significant roles in synaptic function and vesicle trafficking. Alpha-synuclein, in particular, is a major focus due to its association with neurodegenerative diseases, including Parkinson’s disease, where it forms pathological aggregates known as Lewy bodies. 

Content of Synuclein ELISA Kits: Synuclein ELISA kits typically include:

• A 96-well microplate pre-coated with capture antibodies specific to synuclein isoforms (e.g., alpha-synuclein).
• Recombinant synuclein standards for creating a standard curve.
• Detection antibodies, often enzyme-conjugated (e.g., HRP) for signal generation.
• Substrate solution (e.g., TMB – 3,3',5,5'-Tetramethylbenzidine) for colorimetric detection.
• Stop solution to halt the enzymatic reaction.
• Sample and assay diluents, wash buffers, and a detailed protocol for assay preparation and sample handling.

Application of Synuclein ELISA Kits: Synuclein ELISA kits are used in a wide range of research and clinical applications, including:

• Neurodegenerative disease research: Quantifying alpha-synuclein levels in CSF and other biological fluids to study its role in the pathogenesis of Parkinson’s disease, dementia with Lewy bodies, and multiple system atrophy.
• Biomarker discovery: Investigating synuclein as a potential biomarker for early detection and progression monitoring of neurodegenerative diseases.
• Mechanistic studies: Analyzing the role of synucleins in synaptic transmission, vesicle trafficking, and neuron-to-neuron communication.
• Neurotoxicity and aggregation studies: Researching the factors influencing synuclein aggregation and its neurotoxic effects, which contribute to neuronal cell death in Parkinson’s disease.

Synuclein ELISA kits provide sensitive, accurate, and reproducible measurements, making them essential tools for advancing research into the biological functions of synucleins and their implications in neurodegenerative diseases and other conditions.