Tollip ELISA Kits

Tollip (Toll-interacting protein) ELISA kits are immunoassays designed for the quantitative detection of Tollip levels in various biological samples, such as cell lysates, tissue extracts, and serum. Tollip is an adaptor protein that plays a crucial role in modulating innate immune responses, particularly in the Toll-like receptor (TLR) signaling pathways. It is involved in regulating inflammatory responses and maintaining immune homeostasis. The use of Tollip ELISA kits aids in understanding its role in inflammation, immunity, and related diseases, including autoimmune and infectious diseases.

Content of Tollip ELISA Kits: Tollip ELISA kits typically include:

• A 96-well microplate pre-coated with capture antibodies specific to Tollip.
• Recombinant Tollip standards for generating a standard curve.
• Enzyme-conjugated detection antibodies (e.g., HRP) for colorimetric detection.
• Substrate solution, typically TMB (3,3',5,5'-Tetramethylbenzidine), for signal development.
• Stop solution to terminate the enzymatic reaction.
• Sample diluents, assay buffers, wash buffers, and a comprehensive instruction manual for assay preparation and execution.

Application of Tollip ELISA Kits: Tollip ELISA kits are used in various research and clinical applications, including:

• Immunology research: Studying the role of Tollip in modulating TLR-mediated signaling pathways, which are essential for pathogen recognition and activation of innate immunity.
• Inflammatory disease research: Measuring Tollip levels to understand its involvement in the regulation of inflammatory responses, contributing to the study of chronic inflammatory diseases, such as inflammatory bowel disease (IBD) and rheumatoid arthritis.
• Autoimmune disease studies: Investigating Tollip’s role in autoimmunity and its potential modulation in diseases where TLR signaling is dysregulated.
• Infectious disease research: Evaluating Tollip expression in response to infections, as it can act as a negative regulator of TLR signaling to prevent overactivation and excessive inflammation.
• Cancer research: Exploring the role of Tollip in tumor biology, particularly its impact on immune regulation within the tumor microenvironment and its potential involvement in cancer progression.
• Drug discovery and development: Screening for potential therapeutic agents that target Tollip-mediated pathways for the treatment of inflammatory and immune-related disorders.

Tollip ELISA kits provide sensitive and accurate quantification, making them valuable tools for researchers studying the complex roles of Tollip in immune regulation, inflammation, and disease.