Triiodothyronin ELISA Kits
Triiodothyronine (T3) ELISA kits are immunoassays designed for the quantitative measurement of T3 levels in biological samples such as serum and plasma. T3 is one of the two primary thyroid hormones, along with thyroxine (T4), and plays a critical role in regulating metabolism, growth, and development. Accurate measurement of T3 is essential in diagnosing and monitoring thyroid function and related disorders such as hyperthyroidism and hypothyroidism.
Content of Triiodothyronine (T3) ELISA Kits: T3 ELISA kits typically include:
- A 96-well microplate pre-coated with anti-T3 antibodies.
- T3 standards for creating a standard curve to calibrate the assay.
- Enzyme-conjugated detection antibodies (e.g., HRP) specific for T3.
- Substrate solution (e.g., TMB – 3,3',5,5'-Tetramethylbenzidine) for colorimetric signal development.
- Stop solution to terminate the enzymatic reaction.
- Sample and assay diluents, wash buffers, and a detailed instruction manual for assay procedures and sample handling.
Application of Triiodothyronine (T3) ELISA Kits: T3 ELISA kits are widely used in research and clinical diagnostics, including:
- Thyroid function assessment: Measuring T3 levels to assess thyroid health and diagnose disorders such as hyperthyroidism, where T3 is elevated, or hypothyroidism, where it is reduced.
- Endocrinology research: Investigating the role of T3 in metabolic regulation, energy balance, and its broader effects on organs and tissues.
- Monitoring thyroid treatment: Evaluating the efficacy of treatments for thyroid disorders by tracking changes in T3 levels over time.
- Disease correlation studies: Analyzing T3 levels in relation to conditions such as heart disease, where thyroid hormones can affect cardiac function, or metabolic syndromes.
- Pharmacological studies: Assessing the impact of drugs and therapies on thyroid hormone levels and related metabolic pathways.
- Developmental biology: Studying the role of T3 in growth and development, particularly its influence on brain development and maturation.
Triiodothyronine ELISA kits offer high specificity and sensitivity, making them essential tools for obtaining precise measurements of T3 in research and clinical settings. This allows for a deeper understanding of thyroid function and the management of related health conditions.
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