WTAP ELISA Kits

WTAP (Wilms’ tumor 1-associating protein) ELISA kits are immunoassays designed for the quantitative detection of WTAP levels in biological samples such as cell lysates and tissue extracts. WTAP is a regulatory protein that plays a critical role in RNA processing and m6A RNA methylation, influencing gene expression, cell differentiation, and development. 

Content of WTAP ELISA Kits: WTAP ELISA kits typically include:

• A 96-well microplate pre-coated with capture antibodies specific to WTAP.
• Recombinant WTAP protein standards for generating a standard curve.
• Detection antibodies conjugated with an enzyme (e.g., HRP – Horseradish Peroxidase) for colorimetric detection.
• Substrate solution (commonly TMB – 3,3',5,5'-Tetramethylbenzidine) for developing the colorimetric signal.
• Stop solution to terminate the enzymatic reaction.
• Sample and assay diluents, wash buffers, and detailed protocols for sample preparation and assay procedures.

Application of WTAP ELISA Kits: WTAP ELISA kits are used in various research and clinical applications, including:

• Cancer research: Investigating the role of WTAP in tumorigenesis, as it is involved in the regulation of cell proliferation and apoptosis. Overexpression or dysregulation of WTAP has been associated with various cancers, including leukemia and solid tumors.
• Epitranscriptomics: Studying WTAP as part of the m6A RNA methylation complex, where it contributes to the modification and stability of mRNA, affecting gene expression and cell fate decisions.
• Cell cycle regulation: Analyzing WTAP’s involvement in cell cycle progression and its impact on cell division and growth.
• Developmental biology: Exploring WTAP’s role in embryonic development and differentiation, as it is essential for proper cellular development and organogenesis.

WTAP ELISA kits provide accurate and reproducible quantification, making them vital tools for researchers investigating RNA processing, epigenetic regulation, and related cellular mechanisms in health and disease.