AMH ELISA Kits
AMH (Anti-Müllerian Hormone) ELISA kits are immunoassay tools designed for the quantitative measurement of AMH levels in biological samples such as serum, plasma, and follicular fluid. AMH is a glycoprotein hormone produced by granulosa cells in ovarian follicles and plays a crucial role in regulating folliculogenesis and ovarian function. It is commonly used as a marker for assessing ovarian reserve, fertility potential, and various reproductive health conditions.
Content: AMH ELISA kits use the sandwich ELISA format to provide high specificity and sensitivity for AMH detection. Common kit components include:
- 96-well microplate pre-coated with a capture antibody specific to AMH.
- Standards and calibrators for constructing a standard curve to enable accurate quantification.
- Biotinylated detection antibody, which binds to another epitope on the AMH protein.
- Enzyme conjugate (e.g., Streptavidin-HRP) for signal amplification.
- TMB substrate solution, which reacts with HRP to produce a color change.
- Stop solution to halt the enzymatic reaction.
- Wash buffers and assay diluents to ensure consistent performance and reproducibility.
Applications: AMH ELISA kits are widely used in various research and clinical settings, such as:
- Reproductive Health and Fertility Assessments: Measuring AMH levels to evaluate ovarian reserve, predict response to ovarian stimulation in assisted reproductive technologies (ART), and assess fertility potential in women.
- Diagnosis of Ovarian Disorders: Monitoring AMH levels as a marker for polycystic ovary syndrome (PCOS), primary ovarian insufficiency (POI), and other ovarian function abnormalities.
- Oncology Research: Evaluating AMH levels in relation to certain ovarian tumors, as some ovarian neoplasms can alter AMH production.
- Pediatric Endocrinology: Assessing AMH in children to determine gonadal function and evaluate conditions such as disorders of sex development (DSD).
- Menopause Prediction: Utilizing AMH as a biomarker to predict the onset of menopause and track changes in ovarian function over time.
AMH ELISA kits provide reliable and accurate quantification of AMH levels, making them essential for reproductive health assessments and fertility studies. Their application in clinical and research environments contributes significantly to understanding ovarian reserve, diagnosing ovarian disorders, and aiding in fertility planning. Additionally, AMH measurement is valuable in pediatric endocrinology and menopause research, further broadening the scope of its clinical importance.
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