Overview of AMH ELISA Kits

AMH ELISA Kits are designed for the quantitative measurement of Anti-Müllerian Hormone (AMH) levels in various biological samples. AMH is a glycoprotein hormone produced by ovarian granulosa cells and is a key marker for ovarian reserve and fertility assessment.

Technical Content

Key Features:

• High Sensitivity: Capable of detecting low levels of AMH, ensuring accurate measurement even in samples with low hormone concentrations.
• Specificity: High specificity for AMH, minimizing cross-reactivity with other hormones and proteins.
• Wide Range of Applications: Suitable for use with serum, plasma, and other biological fluids.

Applications:

• Fertility Assessment: Used to evaluate ovarian reserve and predict response to fertility treatments in women.
• Reproductive Health Research: Facilitates the study of reproductive aging, polycystic ovary syndrome (PCOS), and other conditions affecting ovarian function.
• Endocrine Research: Assists in understanding the role of AMH in sexual development and endocrine disorders.

Usage Protocol:

• Sample Preparation: Collect and prepare samples according to the kit instructions. Dilute if necessary.
• Assay Procedure: Add samples, standards, and controls to the wells of the ELISA plate pre-coated with AMH-specific antibodies.
• Incubation: Incubate the plate to allow AMH to bind to the antibodies.
• Detection: Add detection antibodies and enzyme substrate to generate a colorimetric signal proportional to the AMH concentration.
• Analysis: Measure the absorbance using a microplate reader and calculate AMH concentrations based on the standard curve.

Benefits:

• Accuracy: Provides reliable and reproducible results for the quantitative analysis of AMH.
• Efficiency: Streamlined protocol with ready-to-use reagents reduces assay time and labor.
• Versatility: Compatible with a variety of sample types, making it suitable for diverse research needs.