Amyloid Beta ELISA Kits

Amyloid beta ELISA kits are designed to detect and quantify Aβ peptides in various biological samples, including cerebrospinal fluid (CSF), plasma, serum, and tissue homogenates. These kits typically employ a sandwich ELISA format, where specific antibodies capture Aβ peptides, and detection is facilitated through enzyme-linked secondary antibodies, resulting in a measurable signal proportional to the Aβ concentration.

Key Components:

• Pre-coated Microplate: Wells pre-coated with anti-Aβ antibodies to capture the target peptide.
• Standards and Controls: Known concentrations of Aβ to generate a standard curve and validate assay performance.
• Detection Antibodies: Enzyme-conjugated antibodies specific to Aβ for signal generation.
• Substrate Solution: Reacts with the enzyme to produce a detectable signal, typically colorimetric.

Applications:

• Alzheimer's Disease Research: Investigating the role of Aβ peptides in AD pathogenesis and progression.
• Clinical Studies: Assessing Aβ levels as potential biomarkers for AD diagnosis and monitoring.
• Pharmaceutical Development: Evaluating the efficacy of therapeutic agents targeting Aβ production or aggregation.

Examples of Amyloid Beta ELISA Kits:

• Human Amyloid Beta 42 ELISA Kit - Invitrogen: Quantifies Aβ42 in human tissue culture medium, tissue homogenate, and CSF, with an assay range of 15.6–1000 pg/mL.
• Human Amyloid beta (aa1-42) Quantikine ELISA Kit - R&D Systems: Measures Aβ42 in cell culture supernates, tissue lysates, and CSF, with a sensitivity of 4.73 pg/mL. 
• Human Amyloid Beta 40 ELISA Kit - Invitrogen: Detects Aβ40 in human tissue culture medium, tissue homogenate, and CSF, with an assay range of 7.8–500 pg/mL.
• LEGEND MAX™ Human Amyloid Beta (1-42) ELISA Kit - BioLegend: Designed for the quantitative measurement of human Aβ42 in various sample types.

By providing reliable and quantitative measurements of Aβ peptides, ELISA kits are indispensable tools in advancing our understanding of Alzheimer's disease and facilitating the development of diagnostic and therapeutic strategies.