ANGPT2 ELISA Kits

ANGPT2 (Angiopoietin-2) ELISA Kits are highly specific immunoassay kits used for the quantitative detection of ANGPT2 levels in various biological samples, such as serum, plasma, cell culture supernatants, and other biological fluids. ANGPT2 is a crucial angiogenic cytokine involved in vascular development and inflammation, and its dysregulation is associated with multiple pathophysiological conditions, including cancer, sepsis, and cardiovascular diseases.

Content of ANGPT2 ELISA Kits

These ELISA kits typically include:

• Pre-coated ELISA Plate: Wells coated with an anti-ANGPT2 capture antibody, ensuring high specificity and sensitivity.
• Standards and Controls: ANGPT2 standards are provided for establishing a standard curve, enabling accurate quantification across samples.
• Detection Antibody: A biotin-labeled or enzyme-linked antibody specific to ANGPT2, allowing for a signal amplification in the presence of ANGPT2 in the sample.
• Substrate Solution: TMB (3,3',5,5'-Tetramethylbenzidine) substrate for colorimetric detection, yielding a color change that is proportional to the ANGPT2 concentration.
• Stop Solution and Wash Buffer: To end the reaction and wash unbound components, ensuring low background noise.

Application of ANGPT2 ELISA Kits

ANGPT2 ELISA Kits are commonly used in:

• Cancer Research: ANGPT2 serves as a biomarker in tumor angiogenesis and metastasis, particularly in cancers like hepatocellular carcinoma, breast cancer, and lung cancer. Elevated ANGPT2 levels can indicate tumor progression and metastatic potential.
• Cardiovascular Disease Studies: ANGPT2 plays a role in vascular remodeling and endothelial dysfunction, making it a significant marker for atherosclerosis, myocardial infarction, and heart failure studies.
• Inflammatory and Infectious Disease Research: ANGPT2 is upregulated in inflammatory conditions, especially in sepsis and systemic inflammatory response syndrome (SIRS), where it is an indicator of endothelial dysfunction and a predictive biomarker for disease severity.
• Diabetes and Metabolic Syndrome: Elevated ANGPT2 levels have been observed in metabolic disorders, serving as a biomarker for endothelial activation and vascular complications associated with diabetes.

By providing precise and reproducible quantification, ANGPT2 ELISA Kits facilitate biomarker discovery, disease progression monitoring, and therapeutic response assessment in research and clinical settings.