APEX ELISA Kits

Apurinic/apyrimidinic endodeoxyribonuclease 1 (APEX1), also known as APE1, is a crucial enzyme in the base excision repair (BER) pathway, responsible for repairing abasic sites in DNA. APEX1 ELISA kits are specialized immunoassays designed to quantitatively measure APEX1 levels in various biological samples, including serum, plasma, cell lysates, and tissue homogenates.

Kit Components:

A typical APEX1 ELISA kit includes:

• Pre-coated 96-well microplate: Wells are pre-coated with a specific capture antibody against APEX1.
• Standards and controls: Known concentrations of APEX1 to generate a standard curve for quantification.
• Detection antibody: Biotinylated antibody specific to a different epitope of APEX1.
• Enzyme conjugate: Streptavidin conjugated to horseradish peroxidase (HRP) for signal amplification.
• Substrate solution: Typically tetramethylbenzidine (TMB), which reacts with HRP to produce a measurable color change.

Applications:

APEX1 ELISA kits are utilized in various research domains:

• DNA Repair Studies: Quantifying APEX1 to understand its role in the BER pathway and its response to DNA damage.
• Cancer Research: Investigating APEX1 expression in tumorigenesis and its potential as a therapeutic target.
• Neurodegenerative Diseases: Exploring APEX1's involvement in neuronal DNA repair mechanisms.
• Pharmacological Research: Assessing the impact of drugs on APEX1 expression and activity.

For detailed information on specific APEX1 ELISA kits, including protocols and performance characteristics, refer to the manufacturer's datasheets.