AURKB ELISA Kits

AURKB ELISA kits are specialized immunoassays designed to detect and quantify Aurora Kinase B (AURKB) levels in various biological samples. AURKB is a serine/threonine kinase that plays a central role in cell division, particularly in chromosome alignment, segregation, and cytokinesis during mitosis. Dysregulation of AURKB has been implicated in tumorigenesis and is associated with poor prognosis in various cancers, making it a significant target in cancer research and cell cycle studies. 

Content of AURKB ELISA Kits

AURKB ELISA kits typically include components optimized for accurate, specific detection and quantification of AURKB:

• Standards and Controls: Calibrated AURKB standards for creating a standard curve, along with control samples to validate assay reproducibility.
• Substrate Solution: Chromogenic or chemiluminescent substrate for enzyme reaction, allowing detection of the signal.
• Stop Solution: Stops the enzymatic reaction for accurate endpoint readings.
• Wash Buffer and Sample Diluent: Buffers to optimize conditions for minimal background interference, ensuring consistent assay performance.

These components are prepared for optimal measurement of AURKB across sample types such as cell lysates, tissue extracts, and serum.

Application of AURKB ELISA Kits

AURKB ELISA kits have significant applications in cancer research, cell cycle studies, and drug development:

• Drug Screening and Development: AURKB is a target for anticancer therapies aiming to disrupt mitotic progression in cancer cells. AURKB ELISA kits are useful for evaluating the impact of therapeutic compounds on AURKB levels, aiding in the identification of potential inhibitors and the development of targeted cancer treatments.
• Biomarker Research: AURKB levels can serve as a biomarker for certain cancers and cell proliferation disorders. By quantifying AURKB expression, researchers can explore its potential as a diagnostic and prognostic marker, helping to improve patient stratification and treatment outcomes.
• Cellular Stress and DNA Damage Studies: AURKB is responsive to DNA damage signals and cellular stress. AURKB ELISA kits can measure its levels in response to treatments or conditions that induce DNA damage, providing data for studies on cellular response pathways.

AURKB ELISA kits are vital tools for advancing understanding of cell division, tumor biology, and the development of targeted therapies, offering precise, reproducible quantification of this crucial mitotic kinase.