BDNF ELISA Kits

BDNF ELISA kits are designed to detect and quantify BDNF concentrations in various biological samples, including serum, plasma, cell culture supernatants, and tissue lysates. These kits typically employ a sandwich ELISA format, where specific antibodies capture BDNF, and detection is facilitated through enzyme-linked secondary antibodies, resulting in a measurable signal proportional to the BDNF concentration.

Key Components:

• Pre-coated Microplate: Wells pre-coated with anti-BDNF antibodies to capture the target protein.
• Standards and Controls: Known concentrations of BDNF to generate a standard curve and validate assay performance.
• Detection Antibodies: Enzyme-conjugated antibodies specific to BDNF for signal generation.
• Substrate Solution: Reacts with the enzyme to produce a detectable signal, typically colorimetric.
• Buffers and Wash Solutions: Ensure optimal reaction conditions and remove unbound substances.

Applications:

• Neuroscience Research: Investigating the role of BDNF in synaptic plasticity, learning, and memory.
• Clinical Studies: Assessing BDNF levels in patients with neurological and psychiatric disorders, such as Alzheimer's disease, depression, and schizophrenia.
• Pharmaceutical Development: Evaluating the impact of therapeutic agents on BDNF expression and function.

Examples of BDNF ELISA Kits:

• Invitrogen™ Human BDNF ELISA Kit: Quantifies human BDNF in serum, plasma, and supernatant samples with a sensitivity of 80 pg/mL.
• R&D Systems Human Free BDNF Quantikine ELISA Kit: Measures human BDNF in cell culture supernatants, serum, and plasma, with a 3.5-hour assay time.
• Abcam Human BDNF ELISA Kit (ab212166): A single-wash 90-minute ELISA designed for the quantitative measurement of human BDNF in various sample types, with a sensitivity of 2.4 pg/mL. 
• Sigma-Aldrich Human BDNF ELISA Kit (RAB0026): Suitable for serum, plasma, cell culture supernatant, and urine samples, with a sensitivity of 80 pg/mL.

By providing reliable and quantitative measurements of BDNF, ELISA kits are indispensable tools in advancing our understanding of neurobiology and facilitating the diagnosis and management of neurological disorders.