CBL ELISA Kits

CBL (Casitas B-lineage Lymphoma) ELISA kits are specialized immunoassay tools designed for the quantitative measurement of CBL protein levels in biological samples, such as serum, plasma, cell lysates, and tissue extracts. CBL proteins are E3 ubiquitin ligases involved in protein degradation, signal transduction, and regulation of cellular functions, including cell growth and differentiation. These kits are essential for research into cellular signaling pathways, cancer biology, and immunology.

Content: CBL ELISA kits typically use a sandwich ELISA format to ensure high specificity and sensitivity for detecting CBL proteins. Common components of these kits include:

• 96-well microplate pre-coated with a capture antibody specific for the CBL protein.
• Standards and controls for creating a standard curve to quantify CBL concentrations.
• Biotinylated detection antibody that binds to an epitope on the CBL protein.
• Enzyme conjugate (e.g., Streptavidin-HRP) for signal amplification.
• TMB substrate solution, which reacts with the enzyme to produce a color change.
• Stop solution to terminate the enzymatic reaction.
• Wash buffers and assay diluents for maintaining consistency and reproducibility in the assay.

Applications: CBL ELISA kits are used in a variety of research fields, including:

• Cancer Research: Investigating the role of CBL proteins in tumor development and progression, as CBL is involved in the regulation of tyrosine kinase signaling and can act as a tumor suppressor or oncogene.
• Signal Transduction Studies: Understanding how CBL mediates protein ubiquitination and degradation within various signaling pathways, including the regulation of receptor tyrosine kinases.
• Immunology: Analyzing CBL's role in immune cell regulation and function, as it contributes to immune response modulation.
• Cell Biology: Studying the involvement of CBL in cellular processes such as proliferation, differentiation, and apoptosis.
• Therapeutic Research: Exploring potential therapeutic interventions that target CBL pathways in diseases characterized by dysregulated ubiquitination, such as cancers and autoimmune disorders.

CBL ELISA kits are valuable tools for researchers studying the role of CBL in cellular signaling and disease mechanisms. By providing accurate and reproducible quantification of CBL protein levels, these kits facilitate advancements in cancer research, immunology, and cell biology.