CD274 ELISA Kits

CD274, commonly known as PD-L1 (Programmed Death-Ligand 1), is an immune checkpoint protein that plays a key role in modulating immune responses, particularly in cancer and autoimmune diseases. CD274 ELISA Kits are highly sensitive and specific immunoassay kits used for the quantitative detection of PD-L1 levels in a range of biological samples, including serum, plasma, and cell culture supernatants. The measurement of PD-L1 is crucial in studies of tumor immunology and immune checkpoint regulation, as PD-L1 is a critical component in immune escape mechanisms utilized by cancer cells.

Content of CD274 ELISA Kits

CD274 ELISA Kits generally include:

• Pre-coated ELISA Plate: Each well is coated with a specific anti-CD274 capture antibody, allowing high specificity for PD-L1 detection.
• Standard and Controls: Serially diluted CD274 standards for creating a standard curve, ensuring reliable and accurate quantification of PD-L1 levels across samples.
• Detection Antibody: Typically a biotinylated or enzyme-conjugated antibody targeting PD-L1, which binds to the captured CD274, amplifying the signal for precise detection.

Application of CD274 ELISA Kits

CD274 ELISA Kits have a broad range of applications, particularly in:

• Cancer Immunotherapy Research: PD-L1 expression is a key target in immune checkpoint blockade therapies, such as anti-PD-1/PD-L1 therapies. Measuring PD-L1 levels in patient samples can aid in assessing therapeutic responses and stratifying patients who may benefit from immune checkpoint inhibitors.
• Tumor Microenvironment Studies: CD274 is expressed in various tumors, often on both tumor cells and tumor-infiltrating immune cells. ELISA-based quantification allows for the monitoring of PD-L1 levels as a potential biomarker for immune evasion and tumor progression.
• Autoimmune Disease Research: CD274/PD-L1 is also implicated in autoimmune diseases, where abnormal immune checkpoint signaling can lead to immune system overactivity. Detecting PD-L1 levels can help in understanding autoimmune pathogenesis and the role of immune checkpoints.

Overall, CD274 ELISA Kits provide accurate, reproducible measurements that are essential for biomarker discovery, treatment monitoring, and understanding PD-L1’s role in various diseases.