CD47 ELISA Kits

CD47 ELISA kits are specialized immunoassays developed for the quantification of CD47, also known as the “don’t eat me” signal, in various biological samples. CD47 is a transmembrane protein that plays a crucial role in immune evasion by inhibiting phagocytosis when interacting with its receptor, SIRPα, on macrophages.

Content of CD47 ELISA Kits

A standard CD47 ELISA kit contains components optimized for the specific and sensitive detection of CD47:

• Standards and Controls: Standardized concentrations of CD47 for calibration, along with control samples to validate assay accuracy and reproducibility.
• Substrate Solution: A chromogenic or chemiluminescent substrate that reacts with the enzyme to produce a detectable signal.
• Stop Solution: Stops the enzymatic reaction, allowing accurate optical density (OD) readings.
• Wash Buffer and Sample Diluent: Provided to optimize assay conditions, minimizing background noise and nonspecific binding.

These components ensure consistent quantification of CD47 across sample types, such as plasma, serum, and cell lysates.

Application of CD47 ELISA Kits

CD47 ELISA kits are critical tools in cancer biology, immunotherapy research, and studies related to immune cell interactions:

• Therapeutic Screening and Development: CD47 is a target for immune checkpoint inhibitors. CD47 ELISA kits facilitate drug screening by allowing researchers to measure CD47 expression levels in response to candidate therapeutic compounds, helping assess their impact on CD47-related signaling.
• Biomarker Discovery and Validation: CD47 is a promising biomarker for various cancers and inflammatory conditions. Measuring CD47 levels can help in biomarker validation efforts, providing insights into patient stratification and treatment response, particularly in immunotherapy settings.
• Signal Transduction and Cell Communication: CD47-SIRPα signaling is essential in cell-to-cell communication, especially in immune cells. CD47 ELISA kits are useful for investigating this pathway’s impact on immune cell behavior, assisting studies on immune tolerance and regulation.

With its vital role in immune response modulation and tumor biology, CD47 ELISA kits are indispensable in oncology and immunology research, helping advance the understanding and development of novel immunotherapies.