CEACAM (Carcinoembryonic Antigen-Related Cell Adhesion Molecule) ELISA kits are used to measure levels of CEACAM proteins in biological samples. CEACAMs are a family of cell adhesion molecules involved in various physiological and pathological processes, including cancer progression, immune response, and tissue development.
Components
- Coated Microplate:
- A 96-well plate pre-coated with antibodies specific to CEACAM. This allows CEACAM proteins in the sample to be captured on the plate.
- Standards:
- Recombinant or purified CEACAM proteins with known concentrations used to create a standard curve for quantification.
- Detection Antibody:
- A secondary antibody specific to CEACAM, typically conjugated to an enzyme such as horseradish peroxidase (HRP) or alkaline phosphatase (AP) for detection.
- Substrate Solution:
- Contains a substrate that reacts with the enzyme linked to the detection antibody, producing a color change proportional to the amount of CEACAM present.
- Stop Solution:
- An acid solution that halts the enzyme reaction and stabilizes the color for measurement.
- Wash Buffer:
- Used to wash away unbound substances and reduce background noise, ensuring that only specifically bound CEACAM is measured.
Technical Considerations
- Sensitivity:
- The lowest concentration of CEACAM detectable by the assay.
- Specificity:
- The ability of the kit to specifically detect CEACAM without cross-reacting with other molecules.
- Reproducibility:
- Consistency of results across different assays and sample conditions.
- Interferences:
- Potential effects of other substances in the sample that might impact the accuracy of the assay.
Applications
- Research: Investigating CEACAM’s role in cell adhesion, cancer biology, and immune responses.
- Clinical Diagnostics: Monitoring CEACAM levels for diagnostic and prognostic purposes in cancer and other diseases related to cell adhesion and immune function.
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